Minelute pcr purification kit using a microcentrifuge. Rna amounts corresponding to less than one cell as little as 1 pg can be concentrated see figure concentration of rna. Qiagen sample and assay technologies qiagen is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. Add 50ul rnasefree water to sample total volume at 100ul.
Rna cleanup and concentration this protocol is designed for cleaning up rna from enzymatic reactions, for desalting rna samples, and for concentrating rna isolated by various methods. Rneasy minelute cleanup handbook harvard university. Minelute columns are designed to give high endconcentrations of purified dna fragments for subsequent reactions. Qiagen minelute pcr purification kit, 50 minelute spin columns, 5g binding capacity, 10l elution volume, tube format, silica technology, 70 bp to 4 kb fragment size, manual processing, dna sample, fast procedure and easy handling, high, reproducible recoveries. The following brief version of the protocol is provided for your convenience. Minelute pcr purification kit 50 from qiagen selectscience. Find out how to transfer protocols to your qiacube. Print bookmark share pdf 323kb english format file size language download get adobe reader contact qiagen. Qiagen kit handbooks and user manuals are available at. The remaining components of the rneasy micro kit should be stored dry at room temperature. The minelute pcr purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other. Qiagen distributors please see the last page for contact information for your local qiagen distributor. Forensic postpcr purification protocol using the minelute pcr purification kit this protocol, adapted from smith and ballantyne 2007, is for forensic postpcr purification using the minelute pcr purification kit. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol.
Qiagen rneasy minelute cleanup kit, 50 preps, 200l sample, 10 to 14l elution volume, crude rna preps sample, total rna purification, silica technology, minelute spin column format, ideal for pcr, qpcr, realtime pcr, microarray, includes 50 rneasy minelute spin columns, collection tubes. Qiagen minelute pcr purification kit, 50 minelute spin columns, 5g binding capacity, 10l elution volume, tube format, silica technology, 70 bp to 4 kb fragment size, manual processing, dna sample, fast procedure and easy handling, high, reproducible recoveries, for. For concentration of total cellular rna purified using the paxgene blood rna kit, see the rna cleanup protocol on page. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. For purification of up to 5 g pcr products 70 bp to 4 kb in low elution volumes.
It is designed to purify doublestranded dna fragments. Processing nondsp qiaamp mini and qiaamp minelute spin columns on the qiavac 24 plus a vacuum pump capable of producing a vacuum of at least 900 mbar e. Rnasefree dnase set box, containing rnasefree dnase, buffer rdd, and rnasefree water, should be stored immediately upon receipt at 28c. Oragenerna purification protocol using the qiagen rneasy. Qiaquick pcr purification kit 28104 pdf click here qiagen 28104 per the qiaquick protocol and elute with 32 il eb warmed to 55 c. Our advanced, highquality products and services ensure success from sample to result. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit. Place ffpe core material into a 2 ml safelock microcentrifuge tube and close the lid. Binding dna to the qiaamp minelute column membrane to allow optimal binding of dna to the membrane, buffer al and in most protocols, ethanol is added to the lysate. For rna cleanup and concentration with small elution volumes. Transfer the sample onto an rneasy minelute spin column in a 2 ml collection tube.
Qiagen provides a variety of qiaamp genomic dna kits for fast, efficient and easy. These are available online in convenient and compact pdf format. I am afraid that any column based clean up procedure rneasy, qiagen would lose. The minelute pcr purification kit provides spin columns, buffers, and collection tubes for silicamembranebased purification of pcr products 70 bp 4 kb in size. Rneasy mini handbook 042006 7 the rneasy mini kit and rneasy protect mini kit are intended as generalpurpose devices. A link to the relevant protocol sheet is provided in the detailed information about the protocol. This protocol is designed for purification of total dna from grampositive bacteria. Rneasy minelute cleanup kit 50, 74204 from qiagen labsave. This product is not intended for the diagnosis, prevention, or treatment of a disease.
If you also use qiaamp minelute virus kits, be aware that the qiagen protease. The rnasefree dnase set is intended for molecular biology applications. Dna purification from blood or body fluids spin protocol. Qiaquick nucleotide removal kit qiaamp minelute virus. Total rna minelute cleanup with dnase digestion using. Qiagen grants no license under any of its intellectual property to use or incorporate the enclosed components of this kit with any components not included within this therascreen kras rgq pcr kit except as described in the therascreen kras rgq pcr kit instructions for use handbook and additional protocols available at 2.
Our technical service departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of qiagen prod ucts. Dna adsorbs to the dneasy membrane in the presence of high concentrations. Qiagen plasmid purification handbook harvard university. Rneasy minihandbook rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup rneasy protect mini kit for immediate stabilization of rna in harvested animal tissues and subsequent total rna purification rneasy plant mini kit for purification of total rna from plants and. Qiaquick gel extraction kit protocol using a microcentrifuge. The purchaser must determine the suitability of the product for its particular use.
No claim or representation is intended for their use to identify any specific. Qiaamp dna mini and blood mini handbook moodle ufsc. Qiaamp mini and qiaamp minelute spin columns are processed on the. For a more refined search, select more than one parameter. The minelute pcr purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from dna. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. For concentration of total cellular rna purified using the paxgene blood rna kit, follow the protocol on. Protocol qiaquick spin handbook 032001 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. What is the difference between rneasy mini spin columns and. The rneasy minelute cleanup kit provides highquality total rna, free from impurities or enzymatic inhibitors, with a 260 a 280 ratios of 1.
All dna genotek protocols, white papers and application notes, are available in the. Hi i have the buffers for the rneasy mini kit and some left over minelute. You can search for protocols and protocol sheets by selecting an application, kit, or starting material. These are available online in convenient and compact pdf format at. Fragments ranging from 100 bp to 10 kb are purified from primers. This protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Minelute handbook 032008 5 storage upon arrival, open the kit and store minelute spin columns at 28c. The spin columns are designed to allow elution in very small volumes as little as 10.
Minelute handbook 062004 11 adsorption to minelute membrane salt and ph dependence the minelute silicagel membrane is uniquely adapted to isolate dna from both aqueous solutions and agarose gels, and up to 5 g dna can bind to each minelute column. The rneasy micro kit is shipped at room temperature 1525c. Protocol qiaquick spin handbook 072002 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Minelute reaction cleanup kit 50 from qiagen sample to. Qiagen genomictips are gravityflow, anionexchange tips that enable purification of dna of up to 150 kb from a wide range of sample types. Australia qiagen pty ltd po box 25 clifton hill victoria 3068 abn 75 072 382 944 orders 0394893666 fax 0394893888 technical 1800243066 canada qiagen inc. Total rna minelute cleanup with dnase digestion using qiagen. The remaining kit components can be stored at room temperature 1525c. Qiacube pure efficiency walkaway spincolumn processing. Rna cleanup this protocol is designed to clean up rna from enzymatic reactions, for desalting rna samples, and for concentration of rna isolated by various methods. A large amount of rna up to 45 g can be purified and is suitable for use in sensitive. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification.
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